Objective: To study the relationship between the preservating time of deep frozen cryopreservating allograft and the biomechanical properties. Method: Specimens of the fibular bones of deep frozen cryopreservation for 1 and 7 years were made into test pieces of torture and compaction.And the bending strength(BS) ,modulus of elasticity(ME) and compact strength(CS) were measured on the INSTRON1195 electronic tensile test machine.And the results were analysised with T test. Results:The 1 year group and the 7 year group had no significant difference in trems of BS(P>0.05) ,ME(P>0.05) and CS(P>0.05). Conclusion: Cryopreservation had no effects on the cortical bone biomechanics ,as long as not contaminated, the cryopreservation will be independent of storage time and the pre- or pro- operation treatments.