Stem tip callus of chrysanthemum treated with mechanical vibration grow more rapidly than non-treatment callus in vitro. Mechanical vibration treatment enhances the nutriment such as P, K, Ca, Mg element absorption rate of callus in culture. In our experiments we found an optimum frequency, the absorption rate is the highest in this frequency. However, with the frequency increasing, the absorption rate begins to decrease. It is shown that the mechanical vibration has the bidirectional effect. In order to explore the mechanism of the biological eftects caused by mechanical vibration, We studied the distribution of Ca2+ sediments with in cells by electron microscopy and found the Ca2+ distribution was notably different between the control group and treatment groups. In this paper, we also preliminarily approach the mechanism of callus proliferation by mechanical vibration.