Objective To repair critical sized meniscal defects in an immunocompetent mammal by tissue engineering approach and to discuss its biomechanical relevance. Methods Fifteen 45 day old Changfeng crossbred pigs were selected as experimental animals. Autologous fibrochondrocytes were obtained from left knee meniscus by modified Klagsbrun's method and were proliferated in vitro to a proper amount. A 1 cm long full layer defect of right medial meniscus was created anterior to the medial collateral ligament. PGA fibrochondrocyte pluronic complex, fibrochondrocyte pluronic complex or PGA only was respectively implanted into the defects. We used intact menisci and untreated meniscal defects as a control group. Samples obtained in 9w, 16w, 25w and 36w respectively were evaluated by macroscopy, histology, biochemistry and biomechanics. Results From the sights of macroscopic morphology, histological structure and Young's modulus (69.5% of normal meniscus at 36w), the PGA cell pluronic complex can form the best quality tissue, which can stabilize the GAG ratio (58.3% of normal cartilage at 36w) in the cartilage of medial femoral condyle. Conclusion Under a favorable mechanical circumstance, autologous tissue engineered fibrocartilage is a promising method to regenerate or reconstruct menisci, thus preventing the knee from degeneration.