Objective To study the biomechanics of allograft bone tissue, and to provide a suitable preservation method for bone allograft. Methods The femora of fresh corpse were gradient frozen (-2℃/min) to -60℃ after the pretreatment. And then put them into the cryopreservation refrigerator of -80℃. One month later, the biomechanical tests were carried on. Results The results of the biomechanical test were close to the data of fresh bone in former research. Conclusion The biomechanical characteristics of gradient freezing and cryopreservation bone allograft were close to the fresh bone. The method of this preservation could provide gist to the intending further research.