Objective To reveal the reaction dynamic mechanism of the adhesive molecules being involved in the fluorescence recovery after photo-bleaching (FRAP) experiment of the "bull’s eye" type’s contact area, and to develop a new method to measure 2D kinetic rates of the adhesion molecules and their retarded diffusion by FRAP experiment. Method For the FRAP experiment of the "bull’s eye" type’s contact area, a coupled diffusion-reaction model of two different adhesive molecular systems is presented firstly, then the physical process of the FRAP is revealed by numerical modeling, and the behavior of the reaction rate and diffusive retarded coefficient in the FRAP is shown by analyzing the numerical results. Results The FRAP of the "bull’s eye" type’s contact area are governed not only by the 2D reaction rate but also by the diffusive retarded coefficient of the adhesive molecules; Firstly, the process of the FRAP is a free molecular diffusive in the edge domain of the "bull’s eye", then the effect of the reaction is more and more significant as time flows, and become a dominant factor. Conclusion The FRAP experiment of the "bull’s eye" type’s contact area can be modeled by our mathematical model, which can also be used to developed a new technique to measure 2D kinetic rates of the adhesion molecules and their retarded diffusion in a stable "bull’s eye" type’s contact area.