Objective During fetal development, trophoblast cells enter endometrial capillaries, migrate against blood stream within the uterine vasculature, and eventually reside within spiral arteries of the human uterus.In this paper, the hypothesis was tested that by regulating the expression of β1 integrin in trophoblast cells, flow-induced shear stress affects the motility of trophoblast cells.Method Early gestation human trophoblast cells were cultured in glass piece and then exposed to steady shear stresses of 0, 75, 150, or 300 Pa for up to 24 h.Then the migration and β1 intergin expression of the cells were measured.Results Result showed that exposure of the cells to shear stress within 24h significantly increased the level of their motility.Shear stress also altered the expression of β1 integrin in trophoblast cell.Conclusion These results suggest that flow-induced shear stress regulates the expression of β1 integrin, in turn affects the motility of trophoblast cells.