Objective A biodynamic modeling method of human musculoskeletal system based on the general Hamilton and Lie group theory was proposed. Method First, the general coordinate and the Lie configuration manifold of human musculoskeletal system were defined. Second, the biodynamic model of human musculoskeletal system was constructed under the general Hamilton theory. In the end, the computer program of this new algorithm was developed. Result The biodynamic equations of human musculoskeletal system based on the general Hamilton and Lie group theory were developed. A weight lifting behavior's model based on this method was given as an example which proved this method valid. Conclusions The biodynamic equations in this paper could model the human musculoskeletal kinematic and kinetic behavior efficaciously and genuinely which provides a new method for human musculoskeletal biodynamic analysis.