Objective To measure the interstitial fluid pressure (IFP) on low hydraulic resistance channel along meridians and observe the difference and fluctuation. Method Low hydraulic resistance points (LHRP) and non LHRP were measured on anesthetized mini pigs by a scanning hydraulic resistance measuring device. The IFP was then measured by wick in needle method on these two regions. Results The stomach meridian, kidney meridian and conceptual vessel meridian on mini pigs were measured. The IFP were significantly lower than non LHRP region on the above three meridians(P<0.05), the differences of which were 1.06，0.70，3.69 mmHg respectively with the total pressure difference of 1.44 mmHg and pressure gradient of 1.44~2.88 mmHg/cm（1 mmHg=0．133 kPa）. Conclusions Among the peripheral subcutaneous tissues, there exists a difference of IFP toward the meridian which may drive the flow of interstitial fluid toward the meridians.