Objective To analyze the axial stiffness of human pelvis that were set with the double support standing posture and subjected to gradient vertical loads. Method Nine intact embalmed cadaver specimens were marked from 1# to 9# according to the test sequence, and then subjected to vertical static loads in the gradient of 100 N from 0 N to 500 N. The load displacement data were collected by using the beam sensor of electronic universal testing machine to calculate the axial stiffness of human pelvis. Results The differences of axial stiffness among individual pelvis were large (P=0.815), ranging from 240 N/mm to 776 N/mm. All the pelvic specimens were divided into three groups by cluster analysis (the first group: 1#、2#、3#、5#、9#, the second group: 4#、6#、8#, the third group: 7#). According to the processed results, there were statistical differences among the groups (P<0.05). The trends of pelvic axial stiffness were different under gradient loads. The axial stiffness of the first and third group, totally six specimens, increased with loads increasing; while the axial stiffness of the second group including three specimens increased first, but then decreased with the loads increasing. Conclusions There are significant individual differences in pelvic axial stiffness and its changing discipline within the physiological range; the cluster analysis can be used to analyze the changing discipline between the load and stiffness of human pelvis; as a whole, the pelvic axial stiffness increased with the loads increasing.