The science of Tuina(Chinese therapeutic massage) with its long history plays an important role in traditional Chinese medicine. Due to its simple operating skill and unique curative effect, Tuina manipulation has a good reputation for treating traumatological diseases and pain syndromes. Although spinal manipulation is widely used in clinic, its basic research is still weak. In this paper, the relationship between manipulative forces and movements, cracking sounds, real-time measurement on intra-stress of lumbar disc during spinal manipulation, as well as deformation and displacement of lumbar canal and changes of vertebral structures in Cartesian coordinate system were discussed, so to provide a more solid experimental reference and theoretical basis for scientific explanation about mechanism of spinal manipulation.