Objective To analyze mechanical properties of cervical spine manipulation with fine adjustment, so as to provide mechanical data for popularization of such manipulation in clinic. Methods The founder, a skilled practitioner and a beginner of fine-adjusting manipulation operated the spinal fine-adjusting manipulation on two cervical vertebral segments of healthy subjects, respectively. The force data of thumbs were collected by FingerTPS Wireless System to analyze force differences between two thumbs while the force of right thumb was in its peak value, as well as regular pattern of the thumb force in relation with time. Results Significant differences were found between the maximum force of two thumbs in each subject (P＜0.001). The process of force application (from trough to peak, T1) was longer than that of force reduction (from peak to trough, T2) when all the subjects operated spinal fine-adjusting manipulation, showing significant differences (P＜0.001). Conclusions There exists the main and auxiliary hand during cervical spine manipulation with fine adjustment, which is actually a process of slow force application and rapid force reduction.