Objective To analyze the influence of microporous parameters on mechanical behavior of bone tissue engineered-scaffolds, and provide references for optimizing the microporous structure design. Methods The finite element models of scaffolds with microporous structures were established by using ANYSYS software. The relationships between porosity and maximum equivalent stress as well as maximum total deformation were calculated. The effects of microporous spacing and diameter on maximum equivalent stress, maximum total deformation and internal strain were compared and analyzed. Results The influence rule of microporous spacing in x and y direction was consistent. With the increase of microporous spacing from 0.6 mm to 2.0 mm, the maximum equivalent stress reduced from 63.1 MPa to 46.3 MPa, the maximum total deformation reduced from 23.8 μm to 21.8 μm, and the proportion of the best strain range increased from 80% to 84%. However, with the increase of microporous spacing in z direction, the maximum equivalent stress increased from 38.3 MPa to 47.8 MPa, the maximum total deformation increased from 20. 8 μm to 22.8 μm, and the proportion of the best strain range fluctuated within the range of 82%-85%. With the increase of microporous diameter in x and y direction from 0.1 mm to 1.0 mm, the maximum equivalent stress increased from 32.4 MPa to 78.4 MPa, the maximum total deformation increased from 19.9 μm to 38.2 μm, and the proportion of the best strain range reduced from 90% to 53%. With the increase of microporous diameter in z direction, the maximum equivalent stress reduced from 58.8 MPa to 37.9 MPa, the maximum total deformation increased from 23.3 μm to 25.9 μm, and the proportion of the best strain range increased from 82% to 87%. Conclusions The greater the porosity and the proportion of the best strain range, the smaller maximum equivalent stress and maximum total deformation would be, the scaffolds would have the better biological and mechanical properties. These results have reference values for design and optimization of scaffold structure.