Objective To analyze the present status, subject and characteristics of biomechanical studies on injury prevention of anterior cruciate ligament (ACL), which has a practical guiding significance in promoting prevention and control of ACL injuries. Methods Biomechanical studies on ACL injury prevention were conducted by scientometrics based on the Web of Science, CNKI database for statistic analysis. Results Cumulative numbers of issued papers on biomechanical studies of ACL injury prevention well complied with the exponential growth rule year by year in general, which mainly focused on sports science, orthopedic science, surgery, rehabilitation and other disciplines. From the countries participating in the studies, the United States of America was unique, followed by Canada, UK and Japan. Timothy E. Hewett contributed the largest number of issued papers among all the authors participated in the studies. Topics of biomechanical studies on ACL injuries could be identified into four groups: risk factors and incidence, ACL injury of the female, neuromuscular training and control, ACL injury assessment. Conclusions Researchers should broaden their research fields, take advantage of the national and local governments to lead the role in the rehabilitation medical treatment, and pay more efforts in biomechanical studies of ACL injury prevention.