Objective To investigate the effect of different aspect ratios on uniaxial tensile mechanical properties of skin strip specimens from the perspective of experimental methodology. Methods The abdominal skin of New Zealand rabbits was taken as the research object. Strip specimens with aspect ratios ranging from 2 ∶ 1 to 6 ∶ 1 were obtained in the direction from the head to the foot (0°) and in the direction perpendicular to it (90°), and uniaxial tensile tests were performed until the specimens were damaged. After experimental data processing, Kirchhoff stress-Green strain data from uniaxial stretch on the abdominal skin of rabbits were obtained and fitted using a one-dimensional Fung model S= cEexp(αE2), so as to obtain the corresponding material parameters c and α, and statistical analysis of the parameters was performed. Results The differences in c values in two directions were not significant (P = 0. 151), and the differences in α values were relatively large ( P = 0. 001). There was no significant difference in parameters c and α between rabbit abdominal specimens with different aspect ratios in both directions ( P > 0. 05 ). Conclusions Different aspect ratios of rabbit abdominal skin specimens in the range from 2 ∶1 to 6 ∶1 had no effects on their uniaxial tensile mechanical properties.