Objective: This study aimed to investigate the effects of wearing high heels of different heights on ankle joint motion control during walking in women with chronic ankle instability (CAI). Methods: The Vicon infrared motion capture system and a three-dimensional force plate were used to synchronously collect kinematic and kinetic parameters within 200 milliseconds before and after foot contact for 20 healthy females and 20 CAI females while walking on flat ground wearing high heels of 1cm, 3cm, 5cm, and 7cm heights. Two-way repeated measures ANOVA was applied to analyze the data statistically. Results: The study shows that there is an interaction effect between group and heel height on the peak inversion angular velocity and peak inversion angle during foot strike. Post-hoc tests revealed that within the healthy group, compared to a 1cm heel, the 5cm (P=0.002) and 7cm (P=0.002) heels had significantly greater peak inversion angular velocity within 200ms before and after foot strike; there were significant differences in peak inversion angle between the 1cm and 5cm (P=0.018), and 7cm (P<0.001) heels. In the CAI group, compared to a 1cm heel, the 5cm (P=0.002) and 7cm (P=0.002) heels had significantly greater peak inversion angular velocity within 200ms before and after foot strike; there were significant differences in peak inversion angle between the 1cm and 3cm (P<0.001), 5cm (P<0.001), and 7cm (P<0.001) heels. There was a significant main effect of height on peak plantarflexion angle (P<0.001), peak external rotation angle (P<0.001), peak external rotation angular velocity (P<0.001), and peak plantarflexion torque (P=0.048) within 200ms before and after foot strike; there was a significant main effect of group on peak eversion torque (P<0.001). Conclusion: Compared to healthy individuals, women with CAI have reduced ankle joint control while walking in high heels. As heel height increases, ankle stability decreases, suggesting that it is advisable to wear high heels of 3cm in height or below.